WWP1 promotes cell proliferation in hepatocellular carcinoma through ubiquitin-degradation EI24

• Published in: Zhōnghuá zhŏngliú zázhì Vol. 42; no. 3; pp. 203 - 209
• Main Authors: Zhang, Q, Huang, A Z, Shen, F J, Yu, J P, Qin, C Z
• Format: Journal Article
• Language: Chinese
• Published: China 23.03.2020
• Subjects: Animals; Carcinoma, Hepatocellular; Cell Line, Tumor; Cell Proliferation; Etoposide; Gene Expression Regulation, Neoplastic; Liver Neoplasms; Mice; Mice, Nude; Ubiquitin; Ubiquitin-Protein Ligases; Hepatocellular carcinoma; WWP1; Protein degradation; Ubiquitin ligase; EI24
• ISSN: 0253-3766
• DOI: 10.3760/cma.j.cn112152-20200226-00130

To screen the interaction proteins of WW domain containing protein 1 (WWP1), and explore the effects of WWP1 and etoposide induced 24 (EI24) on cell proliferation in hepatocellular carcinoma (HCC). Yeast two-hybrid screening system was used to identify the interaction proteins of WWP1. The interaction was further validated by co-immunoprecipitation. WWP1 and EI24 stably over-expressing or deleted HepG2 cells were established by using the lentivirus transduction method. Colony forming assay and cell counting kit-8 (CCK8) assay were performed to identify the effects of WWP1 and EI24 on cell proliferation. In addition, the role of WWP1 in the tumorigenicity of liver cancer was examined by subcutaneous injection of different level of WWP1 expressed HepG2 into nude mice. WWP1 can interact with EI24 and ubiquitin-degrade EI24 protein. The WWP1 and EI24 over-expressing or deleted HepG2 cell lines were successfully generated. Overexpression of WWP1 decreased while knockdown of WWP1 increased the protein level of EI24