Application of transcriptome in time analysis and donor characterization in blood samples
As an effective supplement to the current forensic DNA typing and one of the research hotpots in forensic science, the in-depth mining and characterization of biological evidence can provide rich and reliable clues for case investigation. In this study, the time-dependent variations of transcriptome...
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Published in | Yíchuán Vol. 45; no. 1; p. 52 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
China
20.01.2023
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Subjects | |
Online Access | Get more information |
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Summary: | As an effective supplement to the current forensic DNA typing and one of the research hotpots in forensic science, the in-depth mining and characterization of biological evidence can provide rich and reliable clues for case investigation. In this study, the time-dependent variations of transcriptome were confirmed in
blood samples within 0-168 days and a random forest model was established to realize the classification of blood samples with different TSD (time since deposition). Meanwhile, significant differences were observed in the transcripts of blood samples with different smoking habits and genders within a certain time period.
,
and
were identified as markers for smoking habit identification, while the transcripts for
and
from the non-recombining region of the Y chromosome (NRY) were identified as markers for male sex identification. Thus, this study provides a theoretical foundation and experimental strategy for establishing a transcriptome-based method for characterizing blood sample retention time and donor characteristics in the field of forensic investigation. |
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ISSN: | 0253-9772 |
DOI: | 10.16288/j.yczz.22-293 |