Establishment of a NOD/SCID mouse model with human immune reconstitution bearing human triple-negative breast cancer

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 35; no. 1; p. 56
• Main Authors: Zhang, Qiao-Chu, Li, Xi, Liu, Rui-Lei, Jiang, Hua, Huang, Ze-Nan, Liu, Yu, Tang, Mi, Huang, Yong
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2015
• Subjects: Animals; Disease Models, Animal; Humans; Leukocytes, Mononuclear; Mice; Mice, Inbred NOD; Mice, SCID; Neoplasm Transplantation; Spleen - immunology; Triple Negative Breast Neoplasms - immunology
• ISSN: 1673-4254

To establish a NOD/SCID mouse model with human immune reconstitution and observe its immune response to human triple-negative breast cancer xenograft. Twenty-four NOD/SCID mice without immune leakage were subjected to cyclophosphamide (CTX) treatment 3 days prior to immune reconstitution with human peripheral blood mononuclear cell (PBMC) injection and subcutaneous transplantation of human triple-negative breast cancer MDA-MB-231 cells, CTX treatment and PBMC injection without tumor cell transplantation, MDA-MB-231 cell transplantation only, or no treatments. The tumor growth and immune responses of the mice were observed at regular intervals. Compared with the tumor-bearing mice, the tumor-bearing mice with immune reconstitution showed prolonged incubation period of tumor formation, slower tumor growth rate and increased survival rate. Human IgG and CD3(+) T cells were detected in the peripheral blood of the mice 1 week after human PBMC injection. The percentage of CD3(+) T cells in the spleen cells was 55.3