A molecular epidemiological study of KI polyomavirus and WU polyomavirus in children with acute respiratory infection in Tianjin, China

• Published in: Zhongguo dang dai er ke za zhi Vol. 19; no. 7; pp. 763 - 769
• Main Authors: Lin, Shu-Xiang, Wang, Wei, Guo, Wei, Yang, Hong-Jiang, Ma, Bai-Cheng, Fang, Yu-Lian, Xu, Yong-Sheng
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2017
• Subjects: Acute Disease; Adolescent; Child; Female; Humans; Male; Molecular Epidemiology; Polyomavirus - genetics; Polyomavirus - isolation & purification; Polyomavirus Infections - epidemiology; Respiratory Tract Infections - virology
• ISSN: 1008-8830
• DOI: 10.7499/j.issn.1008-8830.2017.07.007

To investigate the relationship of KI polyomavirus (KIPyV) and WU polyomavirus (WUPyV) with acute respiratory infection in children in Tianjin, China. A total of 3 730 nasopharyngeal secretions were collected from hospitalized children with acute respiratory infection in Tianjin Children's Hospital from January 2011 to December 2013. Viral nucleic acid was extracted, and virus infection (KIPyV and WUPyV) was determined by PCR. Some KIPyV-positive and WUPyV-positive PCR products were subjected to sequencing. Sequencing results were aligned with the known gene sequences of KIPyV and WUPyV to construct a phylogenetic tree. Amplified VP1 fragments of KIPyV were inserted into the cloning vector (PUCm-T) transformed into E. coli competent cells. Positive clones were identified by PCR and sequencing. The nucleotide sequences were submitted to GenBank. In addition, another seven common respiratory viruses in all samples were detected by direct immunofluorescence assay. In the 3 730 specimens, the KIPyV-positive rate