The effects of recombinant porcine amelogenin on human gingival epithelial cells

To investigate the potential effect of recombinant 25kDa porcine amelogenin (rPAm) on attachment, proliferation and migration of primarily cultured human gingival epithelial cells (HGEC). The second passage of HGECs were exposed to different concentrations of rPAm (0, 5, 10, 20μg/mL, respectively)....

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Published inShanghai kou qiang yi xue Vol. 21; no. 3; p. 257
Main Authors Li, Xi-ting, Shu, Rong, Song, Zhong-chen, Zhou, Yan-bin
Format Journal Article
LanguageChinese
Published China 01.06.2012
Subjects
Amelogenin
Animals
Cell Adhesion
Cell Line
Cell Movement
Cells, Cultured
Epithelial Cells
Gingiva
Humans
Swine
Wound Healing
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Summary:To investigate the potential effect of recombinant 25kDa porcine amelogenin (rPAm) on attachment, proliferation and migration of primarily cultured human gingival epithelial cells (HGEC). The second passage of HGECs were exposed to different concentrations of rPAm (0, 5, 10, 20μg/mL, respectively). Proliferation and attachment activities was measured by using cell counting method. Cellular migration was assayed by using an in vitro wound healing model. The data was quantified by the analysis of GraphPad Prism software. rPAm inhibited HGEC attachment in the adhesion assay, the effect was depended on time and rPAm dose. rPAm suppressed the growth rate of HGEC, that was also dose and time dependent. rPAm inhibited the migration ability of HGEC, the concentration of 20μg/mL group had the most significant effect. rPAm significantly inhibit the growth rate, cell adhesion and migration of HGEC, and the effect was dose- and time- dependent.
ISSN:1006-7248