Efficiency of Inducing CIK from Cryopreserved PBMNC by Using Immune Cell SR

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 26; no. 3; p. 894
• Main Authors: Ma, Dong-Lei, Lin, Ke-Jia, Chen, Chen, Wei, Zong-Ke, Wei, Zhi-Zhang, Luo, Xiao-Ling, Wang, Yu-Huan
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2018
• ISSN: 1009-2137
• DOI: 10.7534/j.issn.1009-2137.2018.03.044

To investigate the efficiency of inducing CIK from peripheral blood mononuclear cells(PBMNC) by using immune cell serum replacement(immune cell SR), so as to provide a new strategy for the industrialized production of immune cells. The PBMNC of healthy volunteers were collected, and these cells were thawed after short-term cryopreservation and cultured to induce CIK cells. The cells viability was measured by trypan blue exclusion, the phenotypes were analyzed by flow cytometry, and the cytotoxicity was determined by Calcein-AM/PI double staining. In cryopreserved PBMNC, the control group cells failed to normally proliferate. Cell proliferation ratio was low in 2% SR group in comparison with the fresh group, and the difference was significant (P<0.05), however, differences were not statistically significant between 5% SR and fresh group or between 10% AP and fresh group. CD3 , CD3 CD8 and CD3 CD56 cell subsets were not significantly different before and after cryopreservation (P>0.05). After being cultured, CD