Expressions of SP and NK-1R increase in the posterior horn of the L5－S2 spinal cord in rats with chronic nonbacterial prostatitis

• Published in: Zhonghua nan ke xue Vol. 22; no. 7; p. 602
• Main Authors: Hao, Li-Ya, Liu, Li, Bai, Xian-Guang, Zheng, Xin-Hua
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2016
• Subjects: Animals; Interleukin-10 - metabolism; Interleukin-1beta - metabolism; Interleukin-2 - metabolism; Male; Pain; Prostatitis - metabolism; Prostatitis - physiopathology; Random Allocation; Rats; Rats, Sprague-Dawley; Receptors, Neurokinin-1 - metabolism; Spinal Cord - metabolism; Substance P - metabolism; Tumor Necrosis Factor-alpha - metabolism; substance P; neurokinin-1 receptor; chronic nonbacterial prostatitis
• ISSN: 1009-3591

To investigate the expressions of substance P (SP) and neurokinin-1 receptor (NK-1R) in the posterior horn of the L5－S2 spinal cord in the rat model of chronic nonbacterial prostatitis (CNP) at different time points of modeling. Forty adult male SD rats were randomly divided into four groups of equal number, control, 45 d model, 60 d model, and 90 d model, and proteins were obtained from the prostatic tissue of another 30 rats. The CNP model was made by intraperitoneal injection of 0.5 ml DPT vaccineand intradermal injection of mixed solution of 1 ml prostatein extract and complete adjuvant at a 1∶1 ratio, while the control rats were injected with the same volume of normal saline. At 45, 60, and 90 days after modeling, we measured the paw withdrawal threshold (PWT) of the rats, determined the levels of TNF-α, IL-1β, IL-2, and IL-10 in the prostate tissue by ELISA, observed the histomorphological changes in the prostate by transmission electron and light microscopy, and detected the expressions of SP and NK1-R