Effect of electroacupuncture on cerebral PKC isozyme expression levels in cerebral ischemia-reperfusion rats

• Published in: Zhēncì yánjiū Vol. 37; no. 4; p. 312
• Main Authors: Wang, Zhan-Kui, Zhang, Lian-Cheng, Zhao, Shu-Hua, Zhang, Jin-Wei, Shang, Li-Hua, Zhang, Yu-Lian, Lin, Cui-Ru, Guo, Jia-Kui
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2012
• Subjects: Animals; Brain Ischemia - enzymology; Brain Ischemia - genetics; Brain Ischemia - surgery; Brain Ischemia - therapy; Cerebrum - enzymology; Cerebrum - surgery; Electroacupuncture; Humans; Isoenzymes - genetics; Isoenzymes - metabolism; Male; Protein Kinase C - genetics; Protein Kinase C - metabolism; Rats; Rats, Wistar; Reperfusion
• ISSN: 1000-0607

To observe the effect of electroacupuncture (EA) of "Dingzhongxian" (MS 5) and "Dingpangxian" (MS 8) on the expression of cerebral protein kinase C (PKC) isozymes in local cerebral ischemia reperfusion injury (CI/RI) rats so as to explore its underlying mechanism in protecting ischemic brain tissue. Seventy-two Wistar rats were randomized into normal control (n = 8), CI/RI model (model, n = 32), and EA (n = 32) groups. The later two groups were further divided into 4, 12, 24 and 72 h subgroups, respectively, with 8 rats in each. CI/RI model was established by occlusion of the middle cerebral artery under anesthesia and reperfusion for 4, 12, 24 and 72 h, respectively. EA (1 mA, 2 Hz/15 Hz) was applied to "Dingzhongxian" (MS5) and "Dingpangxian" (MS8) for 10 min every time, and once again every 12 h after modeling. The expression of PKCgamma and PKCdelta in the ischemic cortex tissue was detected using immunohistochemistry. TdT-mediated dUTP Nick-End Labeling was used to detect neuronal apoptosis of the local