Effect of Dichloromethane Extraction Phase of Patrinia Scabiosaefolia Fisch. Stem on Proliferation and Differentiation of K562 Cells

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 31; no. 1; p. 25
• Main Authors: Mi, Le-Yuan, Li, Ke-Jing, Li, Shan, Liu, Ting, Chai, Xiao-Jing, Zhang, Ying, Li, Juan
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2023
• Subjects: Apoptosis; Cell Differentiation; Cell Proliferation; Humans; K562 Cells; Methylene Chloride - pharmacology; Patrinia; cell apoptosis; induced differentiation; K562 cell line; Patrinia scabiosaefolia Fisch. Stem; dichloromethane extraction phase
• ISSN: 1009-2137
• DOI: 10.19746/j.cnki.issn.1009-2137.2023.01.004

To explore the effect of dichloromethane extraction phase of ethanol extract from stem of Patrinia scabiosaefolia Fisch.(DPSS) on proliferation and differentiation of K562 cells and its related mechanism. MTT assay was used to detect the effects of DPSS at 0, 25, 50, 100 and 200 μg/ml on the proliferation of K562 cells at 24, 48 and 72 hours. Flow cytometry was used to analyze the changes of cell cycle and apoptosis at 24 and 48 hours. Wright-Giemsa staining was used to observe the morphological changes of K562 cells. The cell surface antigens CD33 and CD11b were detected by flow cytometry. The proliferation of K562 cells treated with different concentrations of DPSS was inhibited in a time-dose dependent manner ( =-0.96). Cell cycle analysis showed that with the increase of DPSS concentration, cells in G /M phase increased ( =0.88), and cells were blocked in G /M phase. Flow cytometry results showed that with the apoptosis rate of K562 cells was the highest when treated with 200 μg/ml DPSS for 48 h. Morpholo