Distribution characteristics of trimethylamine N-oxide and its association with gut microbiota

To analyze the distribution of trimethylamine N-oxide (TMAO) in healthy adults with different risk factors and explore its association with gut microbiota. We collected fasting blood samples and fresh fecal samples from 181 subjects without atherogenesis in the carotid arteries. Plasma TMAO levels o...

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Published inNan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 36; no. 4; p. 455
Main Authors Wang, Shan, Xia, Geng-Hong, He, Yan, Liao, Shuo-Xi, Yin, Jia, Sheng, Hua-Fang, Zhou, Hong-Wei
Format Journal Article
LanguageChinese
Published China 01.04.2016
Subjects
Adult
Atherosclerosis
Bacteria - isolation & purification
Biomarkers - blood
Cardiovascular Diseases - blood
Chromatography, Liquid
Gastrointestinal Microbiome
Humans
Methylamines - blood
Risk Factors
RNA, Ribosomal, 16S - isolation & purification
Tandem Mass Spectrometry
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Summary:To analyze the distribution of trimethylamine N-oxide (TMAO) in healthy adults with different risk factors and explore its association with gut microbiota. We collected fasting blood samples and fresh fecal samples from 181 subjects without atherogenesis in the carotid arteries. Plasma TMAO levels of the subjects were determined using stable isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS). The fecal DNA was extracted, and the 16S rRNA V4 tags were amplified and sequenced by Illumina HiSeq 2000. The association between TMAO and classical cardiovascular risk factors were analyzed. Gut microbial community structure was analyzed with QIIME, and LEfSe was used to identify the biomarkers. The median (IQR) TMAO level was 2.66 (1.96-4.91) µmol/L in the subjects. TMAO level was significantly correlated with body mass index and operational taxonomic units (OTU). Individuals with high TMAO levels were found to have abundant Clostridiales, Phascolarctobacterium, Oscillibacter, and Alistipes but le
ISSN:1673-4254