Aberrant methylation of NF2, TIMP-3 and THBS1 genes and their diagnostic values in meningiomas

• Published in: Zhong hua yi xue za zhi Vol. 92; no. 41; p. 2889
• Main Authors: Gong, Jie, Zhu, Shu-gan, Wu, Cheng-yuan, Li, Xin-gang, Liu, Yu-guang, Ren, Xiao-hui, Zhang, Yuan
• Format: Journal Article
• Language: Chinese
• Published: China 06.11.2012
• Subjects: DNA Methylation; Humans; Meningeal Neoplasms - genetics; Meningeal Neoplasms - pathology; Meningioma - genetics; Meningioma - pathology; Neurofibromatosis 2 - genetics; Thrombospondin 1 - genetics; Tissue Inhibitor of Metalloproteinase-3 - genetics
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2012.41.003

To explore the functions of NF2, TIMP-3 and THBS1 genes in the tumorigenesis or progression of meningiomas and analyze the values of these genes in early diagnosis, therapy and prognostic evaluation in meningiomas. A total of 66 cases with histological sections of meningiomas, including solitary (SMs, n = 30) and multiple meningiomas (MMs, n = 36), were retrieved from our departmental archives. All cases were regrouped as benign, atypical and anaplastic (malignant) by hematoxylin & eosin staining according to the recently published WHO classification of nervous system tumors. Genomic DNA was extracted from tumor sections and methylation-specific polymerase chain reaction (MSP) performed to detect the CpG methylation status. Normal brain tissue was used as the control group. And then the differences of methylation rate between SMs and MMs tissues and among different subgroups were analyzed by statistical analyses. The results of methylation in different types of meningiomas demonstrated that the rates of NF2,