Effect of qingchang huash recipe on NF-kappaB/Tolls pathway in ulcerative colitis patients and mechanism study

• Published in: Zhongguo Zhong xi yi jie he za zhi Vol. 33; no. 9; p. 1216
• Main Authors: Shen, Hong, Liu, Zhi-Qun, Zhu, Quan, Zhu, Lei, Zhai, Jin-Hai
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2013
• Subjects: Colitis, Ulcerative - metabolism; Colitis, Ulcerative - pathology; Drugs, Chinese Herbal - pharmacology; HT29 Cells; Humans; Inflammation; Interleukin-8 - metabolism; NF-kappa B - metabolism; Signal Transduction - drug effects; Toll-Like Receptor 4 - metabolism
• ISSN: 1003-5370

To observe the effect of Qingchang Huashi Recipe (QHR) on the activation and expressions of nuclear factor kappaB (NF-kappaB), Toll-like receptors (TLRs), and contents of interleukin-8 (IL-8), thus exploring its possible mechanisms for treating ulcerative colitis (UC). The HT-29 cells were induced to inflammation model by tumor necrosis factor-alpha (TNF-alpha) and lipopolysaccharides (LPS). HT-29 cells were divided into 6 groups, i.e., the vehicle control group, the model control group, the sulfasalazine (SASP) group, the high dose QHR group, the middle dose QHR group, the low dose QHR group. Effects on the cell growth were detected by MTT. The chemoattractant of macrophages was observed using Transwell. The expressions of NF-kappaB and TLR4 protein were detected using immune cell fluorescence method. The content of IL-8 was detected by ELISA. The growth of cells were not inhibited in each group. Statistical difference existed in each dose QHR group in inhibiting the chemoattractant of macrophages, reducing