Superoxide dismutase, malondialdehyde and proline as new quality criteria for Schizochytrium sp. fermentation

The aim of our study was to find a novel method to evaluate the strain quality of Schizochytrium sp. HX-308. An acclimatized strain and an original strain were cultivated under normal condition. Meanwhile, the same acclimatized strain was cultured under two different conditions, the optimum conditio...

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Bibliographic Details
Published inWei sheng wu hsüeh pao Vol. 53; no. 2; p. 136
Main Authors Zhang, Li, Ren, Lujing, Hu, Yuanwei, Qu, Liang, Huang, He
Format Journal Article
LanguageChinese
Published China 04.02.2013
Subjects
Fermentation
Malondialdehyde - metabolism
Microalgae - enzymology
Microalgae - genetics
Microalgae - metabolism
Proline - metabolism
Stramenopiles - enzymology
Stramenopiles - genetics
Stramenopiles - metabolism
Superoxide Dismutase - metabolism
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Summary:The aim of our study was to find a novel method to evaluate the strain quality of Schizochytrium sp. HX-308. An acclimatized strain and an original strain were cultivated under normal condition. Meanwhile, the same acclimatized strain was cultured under two different conditions, the optimum condition and the harsh condition. We detected the activity of superoxide dismutase and the contents of malondialdehyde and proline by WST-1 method, thiobarbituric acid test and colorimetry, respectively. The relationship between the three criteria and the fermentation performance of Schizochytrium sp. HX-308 was studied. The acclimatized strain cultured under the optimal fermentation condition had the lowest superoxide dismutase activity (0.025 U/g protein), malondialdehyde (26.20 mmol/g x Fw) and proline contents (0.098 mg/g x Fw). In contrast, the superoxide dismutase activity of the original strain cultured in the harsh conditions was 5 times higher than that of the acclimatized strain, the malondialdehyde and proline
ISSN:0001-6209