Screening of differentially expressed microRNAs in borderline and malignant gastrointestinal stromal tumors

• Published in: Zhonghua bing li xue za zhi Vol. 42; no. 1; p. 20
• Main Authors: Shi, Yuan, Wang, Cui-zhong, Hou, Ying-yong, He, De-ming, Xu, Chen, Liu, Ya-lan, Hu, Qin, Akesu, Sujie, Zeng, Hai-ying, Shen, Kun-tang, Tan, Yun-shan, Zhu, Xiong-zeng
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2013
• Subjects: Adult; Aged; Aged, 80 and over; Cell Transformation, Neoplastic; Down-Regulation; Female; Gastrointestinal Neoplasms - genetics; Gastrointestinal Neoplasms - pathology; Gastrointestinal Stromal Tumors - genetics; Gastrointestinal Stromal Tumors - pathology; Gene Expression Profiling; Humans; Male; Microarray Analysis; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Real-Time Polymerase Chain Reaction; Up-Regulation
• ISSN: 0529-5807
• DOI: 10.3760/cma.j.issn.0529-5807.2013.01.005

Gastrointestinal stromal tumors (GISTs) have a broad spectrum of biological behaviors ranging from benign, borderline and malignant. This study aimed to screen differentially expressed microRNAs (miRNAs) between malignant and borderline GISTs and to investigate the potential role of miRNAs in the malignant transformation of GISTs. Six GIST samples including borderline tumors (n = 3) and malignant tumors (n = 3) were collected based on the clinical and pathological characteristics. Total RNA was extracted, followed by miRNA microarray analysis to screen the differentially expressed miRNAs. The most significantly expressed 4 miRNAs were then chosen for further validation by real-time PCR in 22 additional GIST samples. Direct comparison of malignant group versus borderline group revealed 14 significantly and differentially expressed miRNAs (P < 0.05, with a fold change of < 0.5 or > 2). Five miRNAs were up-regulated and nine were down-regulated in the malignant group. Four miRNAs (miR-221, miR-135b, miR-675(*) a