Expression of high-mobility group box 1 in neonates with sepsis

• Published in: Zhongguo dang dai er ke za zhi Vol. 21; no. 2; pp. 131 - 138
• Main Authors: Zhuo, Su-Yan, Liao, Li
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2019
• Subjects: HMGB1 Protein - genetics; Humans; Infant, Newborn; Leukocytes, Mononuclear; NF-kappa B; Sepsis - genetics; Signal Transduction
• ISSN: 1008-8830
• DOI: 10.7499/j.issn.1008-8830.2019.02.005

To study the expression of high-mobility group box 1 (HMGB1) in neonates with sepsis and its role in the pathogenesis of neonatal sepsis. A total of 62 neonates with sepsis were enrolled as the sepsis group, 66 neonates with local infection were enrolled as the local infection group, and 70 healthy neonates were enrolled as the healthy control group. Serum levels of interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-17 (IL-17), interleukin-23 (IL-23), C-reactive protein (CRP) and procalcitonin (PCT) were measured. The mRNA expression of HMGB1, Toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-κB) and the protein expression of TLR4 and NF-κB in peripheral blood mononuclear cells (PBMCs) were also measured. PBMCs from healthy neonates were divided into 4 groups: control, HMGB1 treatment, HMGB1+TAK-242 (a TLR4 inhibitor) treatment and HMGB1+PDTC (an NF-κB inhibitor) treatment, and the mRNA expression of TLR4, NF-κB and IL-8 and the protein expression of TLR4 and NF-κB were measured. PBMCs from heal