Effect of high glucose on migration of BMSCs through inhibiting CXCR-4

To explore the effect of high glucose on migration of BMSCs through inhibiting CXCR-4. Bone marrow stromal cells (BMSCs) were obtained from the mandible of Wistar rats and stimulated with different concentrations of glucose (5.5, 16.5 mmol/L). The optimum concentration of SDF-1 was evaluated by Tran...

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Published inShanghai kou qiang yi xue Vol. 23; no. 6; p. 646
Main Authors Zhang, Bo, Liu, Na, Gu, Bin, Wu, Hao, Gao, Yu-xuan, Wang, Dong-sheng, Liu, Hong-chen
Format Journal Article
LanguageChinese
Published China 01.12.2014
Subjects
Animals
Cell Movement
Chemokine CXCL12
Glucose
Mesenchymal Stromal Cells
Rats
Rats, Wistar
Receptors, CXCR4
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Summary:To explore the effect of high glucose on migration of BMSCs through inhibiting CXCR-4. Bone marrow stromal cells (BMSCs) were obtained from the mandible of Wistar rats and stimulated with different concentrations of glucose (5.5, 16.5 mmol/L). The optimum concentration of SDF-1 was evaluated by Transwell assay in physiological glucose concentration (5.5 mmol/L). In the optimum concentration of SDF-1 condition, we detected the effect of SDF-1 and AMD3100 on migration of BMSCs in different concentrations of glucose (5.5, 16.5 mmol/L). CXCR-4 protein levels were determined by Western blot. The mRNA expression of CXCR-4 and MMP-2 were tested by RT-PCR. SPSS 11.0 software package was used for statistical analysis. The optimum concentration of SDF-1 was 100 ng/mL. High glucose could inhibit the migration of BMSCs. In different concentrations of glucose, SDF-1 could promote the migration of BMSCs, but AMD3100 could inhibit this promotion. High glucose condition could inhibit the secretion of CXCR-4 and mRNA expressi
ISSN:1006-7248