EFFECT OF BONE MARROW MESENCHYMAL STEM CELLS ON GASTRIC ULCER REPAIRING

• Published in: Zhongguo xiu fu chong jian wai ke za zhi Vol. 29; no. 7; p. 889
• Main Authors: Wang, Guozhong, Li, Chengjun, Fan, Xichao, Li, Bo, Xiao, Wei, Jin, Li
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2015
• Subjects: Animals; Bone Marrow Cells; Fibroblast Growth Factor 2 - administration & dosage; Fibroblast Growth Factor 2 - physiology; Gastric Mucosa - blood supply; Gastric Mucosa - physiology; Hematopoietic Stem Cells; Male; Rats; Rats, Wistar; Stomach Ulcer - physiopathology; Stomach Ulcer - therapy; Vascular Endothelial Growth Factor A - metabolism; Wound Healing
• ISSN: 1002-1892

To explore the ettect and mechanisms of bone marrow mesenchymal stem cells (BMSCs) on healing quality of acetic acid-induced gastric ulcer. Forty-eight clean grade male Wistar rats were used to establish the model of gastric ulcer with acetic acid and were randomly divided into 3 groups after 3 days of modeling, 16 rats each group. After the abdominal cavity was open and stomach was pulled out, no treatment was given in group A, 150 µL phosphate buffered saline (PBS) and 150 µL BMSCs at passage 4+PBS (1 x 10(8) cells/100 µL) were injected into the gastric wall surrounding the ulcer at 5 different points in groups B and C respectively. After 10 days, the ulcer area was measured, the mucosal thickness and the number of dilated glands were tested in the regenerative mucosa by histological method. And the expression of vascular endothelial growth factor (VEGF) was detected at ulcerative margin by immunohistochemical method. The ulcer area in group C was significantly smaller than that of groups A and B (P < 0.01)