Role of the FOXO3a transcription factor in neuronal apoptosis in neonatal rats with hypoxic-ischemic brain damage

• Published in: Zhongguo dang dai er ke za zhi Vol. 15; no. 11; p. 1023
• Main Authors: Li, De-Yuan, Qu, Yi, Li, Jin-Hui, Zhang, Li, Xiong, Tao, Mu, De-Zhi
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2013
• Subjects: Animals; Animals, Newborn; Apoptosis; Apoptosis Regulatory Proteins - analysis; Bcl-2-Like Protein 11; Female; Forkhead Box Protein O3; Forkhead Transcription Factors - genetics; Forkhead Transcription Factors - physiology; Hypoxia-Ischemia, Brain - pathology; Male; Membrane Proteins - analysis; Neurons - pathology; Proto-Oncogene Proteins - analysis; Rats; Rats, Sprague-Dawley
• ISSN: 1008-8830
• DOI: 10.7499/j.issn.1008-8830.2013.11.023

To explore the role and mechanisms of FOXO3a nuclear translocation in neuronal apoptosis after hypoxia-ischemia (HI). One hundred and sixty 10-day-old Sprague-Dawly rats were randomly divided into two groups: HI and sham-operated. The right common carotid artery was ligated followed by hypoxia exposure for 2.5 hours in the HI group. The sham-operated group rats were not subjected to carotid artery ligation or hypoxia treatment. Rat cerebral cortex was collected at 0.5, 2, 4, 8 and 24 hours after hypoxia. Western blot was used to detect expression of total FOXO3a protein, pnuclear and cytoplasmic FOXO3a and Bim. TUNEL staining was used to detect apoptotic cells. The nuclear protein of FOXO3a obviously increased from 0.5 to 24 hours after HI in a time-dependent manner compared with the sham-operated group (P<0.01). On the contrary, cytoplasmic protein evidently decreased from 0.5 to 24 hours in the HI group compared with the sham-operated group (P<0.01). Bim protein increased from 0.5 hour, peaked at 2 hours, s