Identification of three common sandfies in southern Xinjiang with multiplex PCR

Based on the variable part of mtDNA CO I gene sequence, a multiplex PCR method was developed for the identification of the three common sandflies (Phlebotomus longiductus, Ph. wui, and Ph. alexandri) in southern Xinjiang. The results demonstrated that this multiplex PCR method was reliable, and coul...

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Published inZhongguo jishengchongxue yu jishengchongping zazhi Vol. 32; no. 3; p. 186
Main Authors Zhou, Zheng-Bin, Zhang, Yi, Zhu, Huai-Min, Shi, Wen-Qi, Jin, Chang-Fa
Format Journal Article
LanguageChinese
Published China 01.06.2014
Subjects
Animals
DNA, Mitochondrial
Multiplex Polymerase Chain Reaction
Phlebotomus - genetics
Polymerase Chain Reaction
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Summary:Based on the variable part of mtDNA CO I gene sequence, a multiplex PCR method was developed for the identification of the three common sandflies (Phlebotomus longiductus, Ph. wui, and Ph. alexandri) in southern Xinjiang. The results demonstrated that this multiplex PCR method was reliable, and could be used to identify the three Phlebotomus species. The PCR product of CO I gene from Ph. longiductus, Ph. wui and Ph. alexandri was 248, 632, and 395 bp, respectively.
ISSN:1000-7423