Studies on standardization of methods for screening molluscicides in laboratory IV sensitivity of Oncomelania snails from different months to niclosamide

• Published in: Zhongguo xue xi chong bing fang zhi za zhi Vol. 24; no. 1; p. 35
• Main Authors: Li, You-Zi, Xing, Yun-Tian, Li, Hong-Jun, Qu, Guo-Li, Wang, Wei, Wei, Jian-Ying, Liang, You-Sheng, Dai, Jian-Rong
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2012
• Subjects: Animals; Dose-Response Relationship, Drug; Laboratories; Lethal Dose 50; Molluscacides - pharmacology; Niclosamide - analogs & derivatives; Niclosamide - pharmacology; Snails - drug effects; Time Factors; Wettability
• ISSN: 1005-6661

To understand the sensitivity of Oncomelania snails collected from different months to niclosamide, so as to provide the scientific evidence for the standardization of methods for molluscicide screening and efficacy evaluation in laboratory. The snails collected from the marshland of Zhenjiang City, Jiangsu Province month by month from June 2010 to May 2011. After being raised in laboratory for 24 h, the snails were randomly grouped, and then immersed in different concentrations of 50% wettable powder formulation of niclosamide ethanolamine salt at (25 +/- 1) degrees C with a humidity of 60%. The dead snails were identified and counted, and the mortality rate of snails and median lethal concentration (LC50) were calculated. When the snails were immersed in the solutions of niclosamide at concentrations of more than 0.5 mg/L for 24 h, all the snails collected from different months were dead, while 60%-100% of the snail mortality was achieved for 0.250 mg/L niclosamide, 3%-27% for 0.125 mg/L niclosamide, and 3%