AFLP analysis of the genetic diversity of closely related Rhododendron species of the section Rhodorastra (Ericaceae) from Siberia and the Far East of Russia

Using AFLP analysis, the genetic variation in four population samples of Rhododendron ledebourii, three such samples of Rh. dauricum, two samples of Rh. sichotence, and one sample of Rh. mucronulatum was examined. Application of three selective primer pairs provided genotyping of 87 accessions from...

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Bibliographic Details
Published inGenetika Vol. 48; no. 10; p. 1153
Main Authors Tikhonova, N A, Polezhaeva, M A, Pimenova, E A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.10.2012
Subjects
Amplified Fragment Length Polymorphism Analysis - methods
Far East
Genetic Speciation
Genetic Variation
Genetics, Population
Genotype
Phylogeography
Polymorphism, Genetic
Rhododendron - genetics
Russia
Siberia
Far East
Siberia
Russia
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Summary:Using AFLP analysis, the genetic variation in four population samples of Rhododendron ledebourii, three such samples of Rh. dauricum, two samples of Rh. sichotence, and one sample of Rh. mucronulatum was examined. Application of three selective primer pairs provided genotyping of 87 accessions from the four species at 213 loci. An analysis of molecular variance revealed a high level of differences between all four species (F(ST) = 0.172). Populations within the species were also differentiated: for Rh. dauricum F(ST) = 0.245; Rh. ledebourii F(ST) = 0.264; Rh. sichotence F(ST) = 0.034. The highest Nei distance was observed for Rh. mucronulatum with Rh. dauricum and Rh. ledebourii (D = 0.057 and 0.069, respectively). Similarity was observed between the species pairs of Rh. ledebourii-Rh. dauricum (0.032) and Rh. sichotense-Rh. mucronulatum (0.034). The Far Eastern species Rh. sichotense and Rh. mucronulatum were characterized by an elevated level of intrapopulation variation. The data from the AFLP-based analysis of differentiation and variation that were thus obtained were compared with the literature data on the morphological criteria that differentiate the species of interest and those regarding the history of the formation of their ranges. The independence of all the species we examined was confirmed.
ISSN:0016-6758