Protective effect of H2S pretreatment on cerebral ischemia-reperfusion injury and its mechanisms in rats

• Published in: Zhōngguó yīxué kēxuéyuàn xuébào Vol. 35; no. 3; p. 249
• Main Authors: Qin, Hao, Gu, Li-ze, Gao, Li, Guo, Jun
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2013
• Subjects: Animals; Brain Ischemia - metabolism; Free Radicals - metabolism; Hippocampus - metabolism; HSP70 Heat-Shock Proteins - metabolism; Hydrogen Sulfide - administration & dosage; Hydrogen Sulfide - therapeutic use; Male; Random Allocation; Rats; Reperfusion Injury - metabolism; Reperfusion Injury - prevention & control; Superoxide Dismutase - metabolism
• ISSN: 1000-503X
• DOI: 10.3881/j.issn.1000-503X.2013.03.002

To investigate the protective effect of H2S pretreatment after cerebral schemia/reperfusion injury and its mechanisms in rats. The rat model of global cerebral ischemia/reperfusion injury was established by bilateral common carotid arteries occlusion combined with hemorrhagic hypotension.30 rats were randomly divided into four groups(1)sham group(n=5),in which rats received sham surgery only,with their bilateral vertebral artery and bilateral common carotid artery exposed but without ischemia treatment;(2)global cerebral ischemia/reperfusion model group(IR group,n=5),in which the global cerebral ischemia was induced by 10-min occlusion of bilateral common carotid arteries combined with hypotension;(3)H2S pretreatment group(n=15),in which H2S(12,24,48 Μmol/kg)was intraperitoneally injected before operation;(4)NaCl pretreatment group(n=5),in which the rats were intraperitoneally injected with saline 30 minutes before operation.The activities of superoxide dismutase(SOD)and the levels of malondialdeehyde(MDA)in