UPLC-Q-TOF/MS for rapid analysis of chemical constituents in Sanhuang tablets

• Published in: Zhongguo zhongyao zazhi Vol. 42; no. 9; p. 1685
• Main Authors: Liu, Meng-Jiao, Wang, Yun, Li, Ling-Yun, Li, Xiao-Qing, Dai, Ye-Jia, Zhang, Xue, Ma, Yin-Lian, Zhang, Cun, Li, Wen-Lan
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2017
• Subjects: Chromatography, High Pressure Liquid; Drugs, Chinese Herbal - analysis; Phytochemicals - analysis; Tablets; Tandem Mass Spectrometry; Sanhuang tablets; rhubarb; baicalin; HPLC-MS; berberine
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20170223.010

The qualitative analysis method of ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) was established for the chemical constituents in Sanhuang tablets. Waters ACQUITY BEH C₁₈ (2.1 mm×100 mm, 1.7 μm) column was used with 0.1% formic acid solution (A)-0.1% formic acid acetonitrile (B) as the mobile phase for gradient elution. The flow rate was 0.2 mL•min⁻¹; the sample volume was 1 μL and the column temperature was 30 ℃. The high-resolution quadrupole time-flight mass spectrometry was used as detector with electrospray ion source in both positive and negative models, and the dry gas temperature was 325 ℃. Based on the analysis of mass spectrometry and literature reports, 38 compounds were confirmed, including 1 alkaloid, 1 dianthrone compound, 6 tannins, 7 anthraquinone glycosides, 6 anthraquinones and 17 flavonoids. Liquid chromatography-mass spectrometry method is simple, reliable and rapid to identify the chemical compositions of Sanhuang tablets, an