Role of epithelial sodium channel in rat osteoclast differentiation and bone resorption

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 36; no. 8; p. 1148
• Main Authors: Hu, Song-Yan, Jin, Xiao-Dong, Zhang, Hao, Chen, Jun, Yang, Guo-Zhu, Wang, Xiao-Dong, Tang, Lu, Lu, Xing-Yan, Lu, Li, Li, Qing-Nan
• Format: Journal Article
• Language: Chinese
• Published: China 20.08.2016
• Subjects: Animals; Bone Marrow Cells - cytology; Bone Resorption; Cathepsin K - metabolism; Cattle; Cell Differentiation; Epithelial Sodium Channels - metabolism; Macrophage Colony-Stimulating Factor - metabolism; Osteoclasts - cytology; RANK Ligand - metabolism; Rats; Signal Transduction
• ISSN: 1673-4254

To explore the role of epithelial sodium channel (ENaC) in regulating the functional activity of osteoclasts. Multinucleated osteoclasts were obtained by inducing the differentiation of rat bone marrow cells with macrophage colony-stimulating factor (M-CSF) and RANKL. The osteoclasts were exposed to different concentrations of the ENaC inhibitor amiloride, and the expression of ENaC on osteoclasts was examined using immunofluorescence technique. The osteoclasts were identified with tartrate-resistant acid phosphatase (TRAP) staining, and the positive cells were incubated with fresh bovine femoral bone slices and the number of bone absorption pits was counted by computer-aided image processing. RT-PCR was performed to analyze the expression of cathepsin K in the osteoclasts. s Exposure to different concentrations of amiloride significantly inhibited the expression of ENaC and reduced the number of TRAP-positive osteoclasts. Exposure of the osteoclasts to amiloride also reduced the number of bone resorption pit