The effects of H102 on NF-κB signal pathway in brain of transgenic AD mice

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 32; no. 3; p. 193
• Main Authors: Wang, Chao, Yuan, Xiao-Yong, Sun, Feng-Xian, Jiang, Fang, Xu, Shu-Mei
• Format: Journal Article
• Language: Chinese
• Published: China 08.03.2016
• Subjects: Alzheimer Disease; Animals; Brain - drug effects; Caspase 3 - metabolism; Learning; Memory; Mice; Mice, Inbred C57BL; Mice, Transgenic; NF-kappa B - metabolism; Nitric Oxide Synthase Type II - metabolism; Peptides - pharmacology; Signal Transduction - drug effects; H102; transgenic; Alzheimer disease; mice; NF-κB signal pathway
• ISSN: 1000-6834
• DOI: 10.13459/j.cnki.cjap.2016.03.001

To investigate the effects of β-sheet breaker peptide H102 on NF-κB signal pathway in brain of APP/PS1 double transgenic mice. Thirty 8-week-old APP/PS1 double transgenic mice were randomly divided into model group and treatment group. A group of C57BL/6J mice with the same age and background were served as controls ( =15). H102 5 l(5.8 mg/kg) was infused by intranasal administration to mice in H102 treatment group, and equal volume of blank solution of H102 (chitosan, BSA) was given to mice in control group and model group. After 16 weeks, the ability of spatial reference memory was tested by Morris Water Maze. Then immunohistochemistry tests and Western blot technique were used to detect the content of amyloid beta peptide 1-42(Aβ ), nuclear factor-kappa B (NF-κB), inhibitor of NF-κB (IκB), IκB kinase (IKK), the corresponding phosphorylated proteins (p-NF-κB、p-IκB、p-IKK), inducible nitric oxide synthase (iNOS) and cleaved Caspase 3 proteins in mice brain. ①The ability of learning and memory was significantl