Effects of Betulinic acid on apoptosis in human gastric cancer SGC-7901 cells

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 37; no. 5; p. 495
• Main Authors: Ding, Lu, Shao, Shu-Li, He, Meng-Qi, Huang, Xin, Zhang, Wei-Wei, Zhang, Zhen-Zhu
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2021
• Subjects: Apoptosis - drug effects; Cell Line, Tumor; Cell Proliferation; Humans; Pentacyclic Triterpenes - pharmacology; Stomach Neoplasms - pathology; cell apoptosis; betulinic acid; human gastric carcinoma SGC-7901 cells
• ISSN: 1000-6834
• DOI: 10.12047/j.cjap.6091.2021.060

To investigate the effects of betulinic acid on apoptosis of human gastric cancer SGC-7901 cells. The human gastric cancer SGC-7901cells were divided in to 4 groups, and each group was set with 3 replicates. The SGC-7901cells in control group were not treated with betulinic acid; the other 3 experimental groups were treated with betulinic acid at the concentrations of 10, 20 and 30 mg/L, respectively; each group was incubated in a 5% carbon dioxide incubator for 48 h. Laser confocal microscope was used to observe morphological changes of SGC-7901 cells; Flow cytometry was applied to determine apoptosis rate and mitochondrial membrane potential. The mRNA and protein levels of , and were also detected by qRT-PCR and western blot respectively. Compared with the control group, SGC-7901 cells in the treated group at final concentrations of 10, 20 and 30 mg/L shrinked, appeared apoptosis body along with nuclear splitting. The percentage of cells in early and advanced period of apoptosis were markedly increased ( ＜0