Effect of betulinic acid on proliferation of human gastric cancer SGC-7901 cells

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 36; no. 6; p. 628
• Main Authors: Chen, Ya-Nan, Shao, Shu-Li, He, Meng-Qi, Huang, Xin, Zhang, Wei-Wei, Zhang, Zhen-Zhu
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2020
• Subjects: Apoptosis; Cell Line, Tumor; Cell Proliferation; Humans; Pentacyclic Triterpenes; Stomach Neoplasms - drug therapy; Triterpenes - pharmacology; cell proliferation; betulinic acid; cell cycle; SGC-7901 cells
• ISSN: 1000-6834
• DOI: 10.12047/j.cjap.6036.2020.131

Human gastric cancer SGC-7901 cells were treated with betulinic acid(BA)at the concentrations of 0, 10, 20, and 30 μg/ml, and treated with conventional chemotherapeutic drug 5-Fu as a positive control to explore its effect on cell proliferation. Trypan blue and GIEMSA staining method were used to investigate the effect of BA on cell growth inhibition and clone formation. EdU method and flow cytometry were used to explore the proliferation and cell cycle of SGC-7901 cells after treated with BA, respectively. qRT-PCR and Western blot were also applied to determine the mRNA and protein levels of cyclin D1 and cyclin B1. The cell growth inhibition rate was increased after treated with different concentrations of BA in SGC-7901 cells( ＜0.05). After treated for 48 h, BA decreased the clone information and cell proliferation of SGC-7901 cells markedly in dose-and time-dependent manners ( ＜0.01). Flow cytometry analysis showed that BA obviously increased the proportion of SGC-7901 cells in G1 phase but decreased the