Effects of daunorubicin on KG1a cell proliferation and Eps8 expression

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 21; no. 1; p. 49
• Main Authors: Gan, Jin-Ying, Xiong, Wen-Yan, Wang, Lei, Cai, Song-Hao, Li, Yu-Hua
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2013
• Subjects: Adaptor Proteins, Signal Transducing - metabolism; Cell Line, Tumor; Cell Proliferation - drug effects; Daunorubicin - pharmacology; Humans
• ISSN: 1009-2137
• DOI: 10.7534/j.issn.1009-2137.2013.01.011

The aim of this study was to observe the inhibitory effect of daunorubicin on KG1a cells and the expression of Eps8 which is a novel tumor-associated antigen with its full name epidermal growth factor receptor pathway substrate 8 (Eps8), and to explore the effect of daunorubicin on Eps8 expression in KG1a cells at mRNA and protein levels. The KG1a cells were treated with different concentration of daunorubicin for 24, 48, 72 h, then trypan blue staining was used to detect the inhibitory rate of KGla cells, RQ-PCR and Western blot were used to detect Eps8 mRNA and Eps8 protein expression. The results showed that daunorubicin inhibited the proliferation of KG1a cells in a dose and time dependent manner (r = 0.983, P < 0.01). Daunorubicin could reduce the mRNA and protein levels of Eps8 expression in dose and time dependent manners in KG1a cells (r = 0.979, P < 0.05). It is concluded that with the increasing of concentration and time of daunorubicin acting on KG1a cells, the cell proliferative inhibitory effect