Therapeutic effect of adipose tissue-derived stem cells on bleomycin-induced mice of scleroderma

• Published in: Beijing da xue xue bao. Journal of Peking University. Yi xue ban Vol. 48; no. 6; p. 970
• Main Authors: Dou, J L, Bai, L, Pang, C Y, Zhang, W L, Zhang, W, Wang, Y F
• Format: Journal Article
• Language: Chinese
• Published: China 18.12.2016
• Subjects: Adipose Tissue - transplantation; Adult Stem Cells - immunology; Adult Stem Cells - transplantation; Animals; Bleomycin - poisoning; Cytokines - blood; Cytokines - drug effects; Female; Fibrosis - immunology; Fibrosis - therapy; Inflammation - immunology; Inflammation - therapy; Interleukin-17; Interleukin-6; Lung - pathology; Mice; Mice, Inbred C57BL; RNA, Messenger; Scleroderma, Systemic - chemically induced; Scleroderma, Systemic - therapy; Skin - pathology; T-Lymphocytes, Regulatory - drug effects; T-Lymphocytes, Regulatory - immunology; Th17 Cells - drug effects; Th17 Cells - immunology; Transforming Growth Factor beta; Tumor Necrosis Factor-alpha
• ISSN: 1671-167X

To investigate the effects and mechanisms of adipose-derived stem cells (ADSCs) on bleomycin-induced mice of scleroderma. In the study, 24 C57BL/6J female mice were randomly divided into control group, bleomycin(BLM)group, ADSCs (hypodermic injection) group and ADSCs (intravenous injection) group . BLM [2 mg/(kg×d)] was injected into the mice to establish the model of scleroderma. There were 6 mice in each group .The control group mice were injected with normal saline 2 mL/(kg×d) by subcutaneously. The rest of the three groups were injected with BLM. ADSCs groups were injected with ADSCs (2×10 ) subcutaneously and intravenously, respectively. T-helper 17 (Th17) and regulatory T cell (Treg cell) of spleen cells were detected by flow cytometry. The levels of cytokines in the lung tissue and in the serum were detected by real-time fluorescence quantification. Real-time polymerase chain reaction(PCR) and enzyme-linked immuno sorbent assay(ELISA). The pathology change of skin and lung tissue was observed by hemato