Preparation, characterization and Calu-3 cellular uptake of three kinds of poly(b-benzyl-L-amino)block-poly(ethylene glycol) nanoparticles

The aim of this paper is to compare the cytotoxicity and cellular uptake efficiency of three kinds of poly(b-benzyl-L-amino) block-poly(ethylene glycol) nanoparticles (PXA-PEG-NPs) using Calu-3 cells, and select one as a nasal drug delivery vector for curcumin (Cur). Poly(gamma-benzyl-L-glutamate) b...

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Published inYao hsüeh hsüeh pao Vol. 48; no. 4; p. 560
Main Authors Zhou, Yin, Lu, Li-Na, Xin, Xue, Huo, Dong-Feng, Wu, Hong-Bing, Qiu, Ming-Feng
Format Journal Article
LanguageChinese
Published China 01.04.2013
Subjects
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Administration, Intranasal
Anti-Inflammatory Agents, Non-Steroidal - administration & dosage
Anti-Inflammatory Agents, Non-Steroidal - metabolism
Aspartic Acid - chemistry
Aspartic Acid - toxicity
Cell Line, Tumor
Cell Survival - drug effects
Curcumin - administration & dosage
Curcumin - metabolism
Drug Carriers
Ethylene Glycol - chemistry
Ethylene Glycol - toxicity
Humans
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Lysine - chemistry
Lysine - toxicity
Nanoparticles
Particle Size
Polyethylene Glycols - chemistry
Polyethylene Glycols - toxicity
Polyglutamic Acid - analogs & derivatives
Polyglutamic Acid - chemistry
Polyglutamic Acid - toxicity
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Summary:The aim of this paper is to compare the cytotoxicity and cellular uptake efficiency of three kinds of poly(b-benzyl-L-amino) block-poly(ethylene glycol) nanoparticles (PXA-PEG-NPs) using Calu-3 cells, and select one as a nasal drug delivery vector for curcumin (Cur). Poly(gamma-benzyl-L-glutamate) block-poly(ethylene glycol) nanoparticles (PBLG-PEG-NPs), poly(gamma-benzyl-L-lysine) block-poly(ethyleneglycol) nanoparticles (PZLL-PEG-NPs) and poly(gamma-benzyl-L-aspartate) block-poly(ethylene glycol) nanoparticles (PBLA-PEG-NPs) were prepared by emulsion-solvent evaporation method. MTT assays were used to evaluate the cytotoxicity of PXA-PEG-NPs against Calu-3 cells. The cellular uptake of nanoparticles was visualized by an inverted fluorescence microscope and quantified by a flow cytometer. The results indicated that even at high concentration of 2 mg x mL(-1) the three nanoparticles had no cytotoxicity on Calu-3 cells. Compared to the curcumin solution, the three curcumin-loaded PXA-PEG-NPs showed significant
ISSN:0513-4870