A novel phthalazine based highly selective chromogenic and fluorogenic chemosensor for Co(2+) in semi-aqueous medium: application in cancer cell imaging

• Published in: Photochemical & photobiological sciences Vol. 14; no. 2; pp. 439 - 443
• Main Authors: Patil, Smita, Patil, Rahul, Fegade, Umesh, Bondhopadhyay, Banashree, Pete, Umesh, Sahoo, Suban K, Singh, Narinder, Basu, Anupam, Bendre, Ratnamala, Kuwar, Anil
• Format: Journal Article
• Language: English
• Published: England 01.02.2015
• Subjects: Cobalt - analysis; Color; Fluorescence; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; HeLa Cells; Humans; Ions - analysis; Microscopy, Confocal; Models, Chemical; Molecular Imaging - instrumentation; Nitrates - chemistry; Phthalazines - chemical synthesis; Phthalazines - chemistry; Solvents - chemistry; Spectrum Analysis; Water - chemistry
• ISSN: 1474-9092
• DOI: 10.1039/c4pp00358f

A new phthalazine based chemosensor was developed for the highly selective and sensitive detection of Co(2+) in the mixed solvent system, CH3CN-H2O (1 : 1, v/v). In the presence of Co(2+), the colour of the solution changed from yellow to green; the absorption maxima of was red-shifted from 383 nm to 435 nm, and the fluorescence of at 550 nm was significantly enhanced. The sensor showed a detection limit down to 25 nM by forming a complex species with Co(2+) in 1 : 1 stoichiometry. Furthermore, by means of confocal laser scanning microscopy experiments, it has demonstrated that it can be used as a fluorescent probe for monitoring Co(2+) in living cells.