Effect of different intensities of electroacupuncture stimulation on expression of SOCS-3 and PPAR-gamma mRNA in adipose tissues of obesity rats

• Published in: Zhēncì yánjiū Vol. 38; no. 1; p. 31
• Main Authors: Gao, Jie, Tang, Cheng-Lin, Liu, Ren-Jian, Chen, Xiao-Lin, Xie, Hui, Hou, Yi-Xuan, Yu, Min, Liu, Zu-Li
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2013
• Subjects: Acupuncture Points; Adipose Tissue - metabolism; Animals; Disease Models, Animal; Electroacupuncture - instrumentation; Electroacupuncture - methods; Humans; Male; Obesity - genetics; Obesity - metabolism; PPAR gamma - genetics; PPAR gamma - metabolism; Rats; Rats, Sprague-Dawley; RNA, Messenger - genetics; RNA, Messenger - metabolism; Suppressor of Cytokine Signaling 3 Protein; Suppressor of Cytokine Signaling Proteins - genetics; Suppressor of Cytokine Signaling Proteins - metabolism; Up-Regulation
• ISSN: 1000-0607

To observe the effect of electroacupuncture (EA) on the expression of suppressor of cytokine signaling 3 (SOCS-3) and peroxisome proliferator-activated receptor (PPAR)-gamma genes in the epididymis adipose tissue of obesity rats so as to explore its mechanism underlying weight reduction. SD rats were randomized into normal control, obesity model, EA-5 mA and EA-1 mA groups (10 rats/group). EA stimulation (6 Hz/20 Hz, 5 mA or 1 mA) was delivered to the bilateral "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) for 15 min, once everyday for two weeks. The expression of SOCS-3 mRNA and PPAR-gamma mRNA in the epididymis adipose tissue was detected by reverse transcriptase polymerase chain reaction. Compared with the normal group, the body weight and expression of SOCS-3 and PPAR-gamma mRNA in the model group were up-regulated obviously (P < 0.01). Compared with the model group, the body weight and expression of SOCS-3 and PPAR-gamma mRNA were significantly decreased in the EA group, with the effects of EA-5 mA being bet