Proliferation effect of ligamentum flavum cells induced by transforming growth factor β 1 and its effect on connective tissue growth factor

• Published in: Zhongguo xiu fu chong jian wai ke za zhi Vol. 33; no. 7; p. 883
• Main Authors: Lu, Changhuai, Liu, Zhijun, Zhang, Hongbo, Duan, Yang, Cao, Yanlin
• Format: Journal Article
• Language: Chinese
• Published: China 15.07.2019
• Subjects: Cell Proliferation; Collagen Type I - genetics; Collagen Type I - metabolism; Collagen Type III - genetics; Collagen Type III - metabolism; Connective Tissue Growth Factor - physiology; Humans; Ligamentum Flavum - physiology; Transforming Growth Factor beta1 - physiology; Connective tissue growth factor; lumbar spinal stenosis; ligamentum flavum cells; transforming growth factor β1
• ISSN: 1002-1892
• DOI: 10.7507/1002-1892.201812016

To investigate the effect of transforming growth factor β (TGF-β ) induced proliferation of ligamentum flavum cells and ligamentum flavum hypertrophy and its effect on connective tissue growth factor (CTGF) expression. The ligamentum flavum tissue in lumbar intervertebral disc herniation was extracted and the ligamentum flavum cells were isolated and cultured by collagenase pre-digestion method. Morphological observation, immunofluorescence staining observation, and MTT assay were used for cell identification. The 3rd generation ligamentum flavum cells were divided into 5 groups. The cells of groups A, B, C, and D were respectively sealed with 3 ng/mL TGF-β , 50 ng/mL CTGF, 3 ng/mL TGF-β +CTGF neutralizing antibody, and 50 ng/mL CTGF+CTGF neutralizing antibody. Serum free DMEM was added to group E as the control. MTT assay was used to detect the effects of TGF-β and CTGF on the proliferation of ligamentum flavum cells. Western blot was used to detect the expression of CTGF protein. Real-time fluorescence quan