The intervention effects of AdipoRon on renal injury in type 2 diabetic mice

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 34; no. 6; p. 568
• Main Authors: Huang, Ling, Qu, Xiao-Hu, Chen, Hui, Xie, Ke-Jian, Xiao, Min
• Format: Journal Article
• Language: Chinese
• Published: China 08.06.2018
• Subjects: kidney; AdipoRon; type 2 diabetes; insulin receptor; mice
• ISSN: 1000-6834
• DOI: 10.12047/j.cjap.5587.2018.127

To study the effects of adiponin receptor agonist (AdipoRon) on renal injury in type 2 diabetic mice. The experiment was carried out on 40 SPF C57/BL6 male mice and they were randomly divided into normal control group ( =10) and experimental group ( =30). Mice in experimental group were given with high sugar and high fat feed in combination with only an intraperitoneal injection of small dose of streptozotocin to build the model of type 2 diabetes (T2DM), which were randomly divided into three groups, model control group (DM), low dose AdipoRon group (DM + L) and high dose AdipoRon group (DM+H)( =10). Then the change of blood glucose was detected. The serum levels of insulin receptor (INSR), insulin receptor substrate-1 (IRS-1) and tumor necrosis factor-α (TNF-α) in mice were measured by ELASA. Pathological changes of renal tissues were observed with a light microscope after HE staining. The expressions of pancreatic duodenal homebox-1 (PDX-1) and insulin mRNA in renal tissues were detected by RT-PCR. The con