Hypoxemia induced the changing structure of the lung tissue in SD rat though changing blood clotting and the effects of breviscapine's intervention

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 45; no. 4; p. 567
• Main Authors: Huang, Jian-Ge, Xie, Min, Zhang, Xu, He, Qiu-Ying, He, Gao-Yan
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2014
• Subjects: Animals; Blood Coagulation; Bronchoalveolar Lavage Fluid; Flavonoids - pharmacology; Hypoxia - pathology; Lung - pathology; Plasminogen Activator Inhibitor 1 - metabolism; Protein C - metabolism; Rats; Rats, Sprague-Dawley; RNA, Messenger; Signal Transduction; Transforming Growth Factor beta1 - metabolism
• ISSN: 1672-173X

To investigate the expressions of plasminogen activator inhibitor-1 (PAI-1), activated protein C (APC) and the histology structures of the rat lung tissues in the different hypoxia time; and to investigate the effects of breviscapine to the above changes. Eighty SD rats were randomly divided into A (control), B (hypoxia), C (hypoxia + low-dose breviscapine) and D (hypoxia + high-dose breviscopine) groups with 20 rats in each group. Each hypoxia group placed daily pressure (101 kpa, 10% O2) environment for 8 h, low-dose and high-dose breviscapine groups were given of 10 mg/kg, 40 mg/kg breviscapine by intraperitoneal injection. On the 3rd, 7th, 14th and 21st d, 5 rats were randomly taken from each group and were killed for examination. The hematoxylin and eosin stain (HE stain) was performed for observation on pathological changes in the rat lung tissues. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was performed for detection of the mRNA levels of transforming growth factor (TGF-beta1) an