Production of extracellular proteinases (protein C activators in blood plasma) by the micromycete Aspergillus ochraceus during submerged and solid-state cultivation

The conditions for the submerged and solid-state cultivation of the micromycete Aspergillus ochraceus VKM F-4104D, producing extracellular proteinases that activate protein C in human blood plasma, were optimized. It is shown that the protein C-activating activity of the micromycete in a solid-state...

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Published inPrikladnaja biohimija i mikrobiologija Vol. 49; no. 6; p. 580
Main Authors Osmolovskiĭ, A A, Kreĭer, V G, Baranova, N A, Kurakov, A V, Egorov, N S
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.11.2013
Subjects
Aspergillus - enzymology
Fungal Proteins - chemistry
Fungal Proteins - secretion
Humans
Peptide Hydrolases - chemistry
Peptide Hydrolases - secretion
Plasma - chemistry
Protein C - chemistry
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Summary:The conditions for the submerged and solid-state cultivation of the micromycete Aspergillus ochraceus VKM F-4104D, producing extracellular proteinases that activate protein C in human blood plasma, were optimized. It is shown that the protein C-activating activity of the micromycete in a solid-state culture was 1.5-3.5 times higher than in a submerged culture (as calculated per milliliter of culture medium). Among the extracellular proteins secreted by A. ochraceus VKM F-4104D during submerged and solid-state cultivation, a protein C-activating proteinase with a pI of 6.0-6.3 was identified.
ISSN:0555-1099
DOI:10.7868/s0555109913060147