Oxidative damage of volatile oil from Chenopodium ambrosioides on Vicia faba root tip cells

• Published in: Ying yong sheng tai xue bao Vol. 23; no. 4; p. 1077
• Main Authors: Hu, Wan-Jun, Ma, Dan-Wei, Wang, Ya-Nan, Zhang, Hong
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2012
• Subjects: Apoptosis - drug effects; Chenopodium - chemistry; Lipid Peroxidation; Oils, Volatile - isolation & purification; Oils, Volatile - pharmacology; Oxidative Stress; Pheromones - pharmacology; Plant Roots - cytology; Plant Roots - drug effects; Plant Roots - metabolism; Vicia faba - drug effects; Vicia faba - metabolism
• ISSN: 1001-9332

Chenopodium ambrosioides is an invasive species, which has strong allelopathic effect on surrounding plants. In this study, the methods of soil culture and filter paper culture were adopted to simulate the eluviation and volatilization of the volatile oil from C. ambrosioides, respectively, and to investigate the allelopathy of the volatile oil on the lipid peroxidation and antioxidant enzyme activities of Vicia faba root tip cells, with the mechanisms of the induced tip cell apoptosis analyzed. At the early stage (24 h) of soil culture and filter paper culture, the superoxide dismutase, peroxidase and catalase activities of the tip cells decreased after an initial increase with the increasing dose of the volatile oil, and the malondialdehyde content of the tip cells increased with the increasing volatile oil dose and treated time. At the midterm (48 h) and later (72 h) stages of soil culture and filter paper culture, a typical DNA ladder strip appeared, suggesting that the volatile oil from C. ambrosioides c