Effect of different concentrations of hypertonic sodium fluid resuscitation on intestine injury in rats at the early stage of severe burn

• Published in: Zhonghua wei zhong bing ji jiu yi xue Vol. 30; no. 3; p. 198
• Main Authors: Sun, Yexiang, Yuan, Chunyu, Chen, Xulin, Wang, Fei, Sun, Congsong, Gao, Zhi, Liu, Sheng
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2018
• ISSN: 2095-4352
• DOI: 10.3760/cma.j.issn.2095-4352.2018.03.002

To investigate the effect of different concentrations of hypertonic saline solution (HS) on intestine injury in rats at the early stage of severe burn. 104 adult healthy female Sprague-Dawley (SD) rats were randomly divided into five groups: sham group (n = 8), lactated Ringer solution (LR) group (n = 24) and 200, 300, 400 mmol/L HS group (HS200 group, HS300 group, HS400 group, all n = 24). All the rats in LR group and different concentrations of HS groups were scalded for 30% total body surface area (TBSA) with III degree, after immediately, the rats were given burn resuscitation therapy by LR or corresponding concentrations of HS through the tail vein. Eight rats were sacrificed on the 2nd, 8th and 24th post-injury hour (PIH), respectively, to collect abdominal aorta blood and intestinal tissues. The rats in sham group were given simulation of burns without resuscitation, which were immediately sacrificed and the specimens were harvested. The serum Na concentration was determined by automatic biochemical an