Analytical studies on flavonoids constituents in Apocynum venetom leaves by UPLC-Q-TOF-MS

To establish an ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometric (UPLC-Q-TOF-MS) method for analyzing the flavonoids constituents in Apocynum venetom leaves. The analysis was performed on an Alltima C18 analytical column with a gradient solvent system of acetonitr...

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Bibliographic Details
Published inZhong yao cai Vol. 37; no. 7; p. 1199
Main Authors Song, Jian-Ping, Xu, Hu, Chen, Fei, Liu, Xun-Hong, Zhang, Feng-Su, Hou, Ya, Ma, Yang
Format Journal Article
LanguageChinese
Published China 01.07.2014
Subjects
Acetonitriles
Apocynum - chemistry
Chromatography, High Pressure Liquid - methods
Flavonoids - analysis
Mass Spectrometry - methods
Plant Leaves
Solvents
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Summary:To establish an ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometric (UPLC-Q-TOF-MS) method for analyzing the flavonoids constituents in Apocynum venetom leaves. The analysis was performed on an Alltima C18 analytical column with a gradient solvent system of acetonitrile-1.0% acetic acid at the flow rate of 0.8 mL/min. The detection wavelength was at 254 nm and the column temperature was at 30 degrees C. Identification and detection were collected under the negative electrospray ion mode using Q-TOF-MS. 11 flavonoids constituents being separated and identified, six of them were detected simultaneously. The results showed that the six detected flavonoids constituents had good linearity between concentrations and peak area (r > 0.9996), and the average recoveries of the method were between 94.3% - 103.3%. The method is accurate, reproducible, and can be used for the quality control of Apocynum venetom leaves.
ISSN:1001-4454