Synergistic Mechanism of Interferon alpha-1b, Interleukin-2 and Thalidomide for Immune Regulation in Patients with Acute Myeloid Leukemia

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 29; no. 1; p. 26
• Main Authors: Mi, Rui-Hua, Chen, Lin, Zhou, Ya-Lan, Li, Dong-Bei, Liu, Sha, Wang, Xiao-Jiao, Liu, Jia, Wang, Min-Fang, Ma, Xiao-Miao, Li, Zhi-Chun, Zhao, Hong-Mian, Xu, Yu-Lin, Chen, Shu-Xia, Yang, Hai-Ping, Guo, Zhi-Qiang, Luan, Chun-Lai, Guo, Shu-Li, Song, Qing-Lin, Wei, Xu-Dong
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2021
• Subjects: CD8-Positive T-Lymphocytes; Humans; Interferon-alpha; Interleukin-2; Leukemia, Myeloid, Acute - drug therapy; Perforin; Thalidomide
• ISSN: 1009-2137
• DOI: 10.19746/j.cnki.issn.1009-2137.2021.01.004

To explore the synergistic immunomodulatory mechanism of interferon alpha-1b, interleukin-2 and thalidomide (ITI) regimen on patients with acute myeloid leukemia (AML). Sixty eight untreated de novo or relapsed or refractory or maintenance therapy patients with AML admitted in the Affiliated Cancer Hospital of Zhengzhou University and the other 11 medical units from March 2016 to May 2019 were treated with ITI regimen. Peripheral blood specimen per patient was collected into EDTA-K3 anticoagulation vacuum tube before the administration of ITI and 3 months after the treatment; peripheral blood lymphocyte subsets and perforin and Granzyme B expression were analyzed by using flow cytometry; the levels of VEGF, IFN-γ, TNF-α and IL-6 in the plasma were detected by using a cytometric bead array. Thirty-five healthy subjects from the hospital physical examination centre were selected as normal controls. The ratio of CD4 /CD8 T cells, the percentage of NK cells, the expression of perforin and Granzyme B of NK cells i