Effect of cyclosporine A on TGF-β/Smad3 signaling in rat gingival fibroblasts

The aim of the study was to investigate the effect of cyclosporine A (CsA) on TGF-β/Smad3 signaling in rat gingival fibroblasts and to explore the mechanism of CsA induced gingival overgrowth. Healthy Sprague-Dawley rat gingival fibroblasts were cultured with different concentrations of CsA and the...

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Published inShanghai kou qiang yi xue Vol. 24; no. 5; p. 557
Main Authors Chen, Jia-lu, Yu, You-cheng, Gong, Yi-ming, Yang, Fei
Format Journal Article
LanguageChinese
Published China 01.10.2015
Subjects
Animals
Cell Proliferation
Collagen
Cyclosporine - pharmacology
Enzyme Inhibitors - pharmacology
Fibroblasts - metabolism
Gingiva - metabolism
Gingival Overgrowth
Rats
Rats, Sprague-Dawley
RNA, Messenger
Smad3 Protein - metabolism
Transforming Growth Factor beta - metabolism
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Summary:The aim of the study was to investigate the effect of cyclosporine A (CsA) on TGF-β/Smad3 signaling in rat gingival fibroblasts and to explore the mechanism of CsA induced gingival overgrowth. Healthy Sprague-Dawley rat gingival fibroblasts were cultured with different concentrations of CsA and the cell proliferations were assessed with CCK-8 assay. The mRNA levels of TGF-β1, Smad3 and collagen I were measured by real-time PCR. The protein level of TGF-β1, Smad3, p-Smad3 and collagen I were determined using western blot and immumofluorescence. Cell migration ability was detected by cell wound scratch assay. The data was analyzed with SPSS 20.0 software package. The use of 200 ng/mL CsA stimulated proliferation and migration of gingival fibroblasts. The mRNA levels of TGF-β1 and collagen I were significantly promoted after CsA exposure. The protein syntheses of TGF-β1, p-Smad3 and Collagen I were also increased by CsA stimulation. CsA may activate TGF-β/Smad3 signaling pathway, thus promoting the proliferation
ISSN:1006-7248