Protective effects of pomegranate polyphenols on cardiac function in rats with myocardial ischemia/reperfusion injury

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 32; no. 7; p. 924
• Main Authors: Dong, Shuying, Tong, Xuhui, Liu, Hao, Gao, Qin
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2012
• Subjects: Animals; Creatine Kinase - blood; Heart - drug effects; L-Lactate Dehydrogenase - blood; Lipid Peroxidation; Male; Malondialdehyde - blood; Myocardial Reperfusion Injury - metabolism; Myocardial Reperfusion Injury - physiopathology; Myocardium - metabolism; Oxidative Stress - drug effects; Polyphenols - pharmacology; Punicaceae; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species - metabolism; Superoxide Dismutase - metabolism
• ISSN: 1673-4254

To determine the protective effect of pomegranate polyphenols on cardiac function in rats with myocardial ischemia/reperfusion (I/R) injury and explore the possible mechanism. Fifty SD rats were randomized into 5 equal groups, including a sham-operated group, an I/R model group, and 3 pomegranate polyphenol dose groups (150, 300, and 600 mg/kg). The rats were subject to a 45-min left main coronary artery occlusion followed by a 180-min reperfusion to induce myocardial I/R injury except for the rats those in the sham-operated group. The cardiac functions were monitored continuously during the experiment. At the end of the reperfusion, arterial blood samples were obtained to measure plasma contents of lactate dehydrogenase (LDH), creatine kinase (CK), malondialdehyde (MDA) and the activities of superoxide dismutase (SOD). Triphenyltetrazolium chloride (TTC) staining was used to evaluate the infarct size. Compared with the sham-operated group, all the rats with I/R showed significantly decreased left ventricular