m-Nisodipine inhibited 5-HT-induced proliferation of rat PASMCs through Rho/ROCK signal pathway

• Published in: Yao hsüeh hsüeh pao Vol. 50; no. 7; p. 824
• Main Authors: Liu, Huan-long, Yu, Ding, Zhu, Zhong-ning, Su, Su-wen, Chen, Xue-yan, Zhang, Yong-jian
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2015
• Subjects: Animals; Cell Proliferation - drug effects; Myocytes, Smooth Muscle - cytology; Myocytes, Smooth Muscle - drug effects; Nisoldipine - pharmacology; Protein Phosphatase 1 - metabolism; Pulmonary Artery - cytology; Rats; rho-Associated Kinases - metabolism; rhoA GTP-Binding Protein - metabolism; Serotonin - pharmacology; Signal Transduction
• ISSN: 0513-4870

This paper is to report the exploration of the activation of Rho/ROCK signal pathway in 5-HT-induced proliferation of rat pulmonary artery smooth muscle cells (PASMCs) and the inhibitory effect of m-Nis on this pathway. PASMCs were cultured with the explant technique. MTT assay was used to explore the proliferation of PASMCs after 5-HT treated for different time and the intervening effect of m-Nis. RT-PCR and Western blot were used respectively to explore the mRNA expression of RhoA, ROCK1 and the protein expression of p-MYPT1 in 5-HT-treated PASMCs and intervening effect of m-Nis. The results of MTT assay suggested that 5-HT (1 µmol · L(-1)) treatment for 12-72 h significantly induced the proliferation of rat PASMCs (P<0.05 or P < 0.01), which were inhibited by m-Nis (1 x 10(-5), 1 x 10(-6), l x 10(-7), 1 x10(-8) mol · L(-1)) in dose-dependent manners (P < 0.05 or P < 0.01). Similarly, the mRNA expression of RhoA, ROCK1 and the protein expression of p-MYPT1 were also inhibited by m-Nis in different degrees (