The Molecular Mechanism of LncRNA HOTAIR Regulating the Proliferation, Invasion and Migration of Lymphoma Cells through Target Gene miR-20a-5p

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 31; no. 1; p. 89
• Main Authors: Lei, A-Ming, Zhu, Ping-An, Fu, Li-Mei, Liao, Xin, Azi, Gu-Li, Zhu, Ping
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2023
• Subjects: Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation; Gene Expression Regulation, Neoplastic; Humans; Lymphoma; MicroRNAs - genetics; RNA, Long Noncoding - genetics; RNA, Small Interfering; HOTAIR; migration; invasion; proliferation; miR-20a-5p; lymphoma
• ISSN: 1009-2137
• DOI: 10.19746/j.cnki.issn.1009-2137.2023.01.014

To investigate the effects of lncRNA on the proliferation, invasion and migration of lymphoma cells through target gene and its molecular mechanism. After synthesizing siRNA and siRNA NC plasmids, they were transfected into lymphoma Raji cells, respectively. The expression of mRNA was detected by RT-qPCR. The proliferation, invasion and migration of lymphoma Raji cells were detected by CCK-8 assay, Transwell assay and cell scratch healing assay, respectively. The target gene of lncRNA was predicted by miRcode software, and the relationship between and target gene was analyzed by dual luciferase assay. After synthesis of inhibitor and inhibitor NC, Raji cells were transiently transfected. The expression of was detected by RT-qPCR, and the effects of down-regulation of on the proliferation, invasion and migration of Raji cells were analyzed. The overexpression plasmid of lncRNA and mimics were transfected into Raji cells simultaneously to analyze the proliferation, invasion and migration ability of Raji cells.