Ectopic osteogenesis of stromal cell-derived factor 1 combined with simvastatin-loaded collagen scaffold in vivo

• Published in: Beijing da xue xue bao. Journal of Peking University. Yi xue ban Vol. 47; no. 1; p. 47
• Main Authors: Ou, Meng-reng, Zhang, Xiao, Liu, Yun-song, Ge, Yan-jun, Zhou, Yong-sheng
• Format: Journal Article
• Language: Chinese
• Published: China 18.02.2015
• Subjects: Animals; Bone Substitutes - chemistry; Chemokine CXCL12 - pharmacology; Collagen - chemistry; Mice; Mice, Inbred ICR; Minerals - chemistry; Osteocalcin - metabolism; Osteogenesis; Osteopontin - metabolism; Simvastatin - pharmacology; Skull; Tissue Engineering
• ISSN: 1671-167X

To construct and evaluate a novel tissue-engineered bone composed of murine stromal cell-derived factor 1(mSDF-1), simvastatin (SIM) and collagen scaffold (Bio-Oss®), serving as a cell-homing approach for bone formation. In the study, 32 ICR mice were randomly divided into 4 groups,each group including 8 mice. The drug-loaded collagen scaffolds were implanted subcutaneously onto the cranium of each mouse according to the groups: (1) 1:50 (volume ratio) dimethyl sulfoxide (DMSO)/phosphate-buffered saline (PBS) solution + collagen scaffold (blank control group); (2) 10⁻³ mol/L SIM solution + collagen scaffold (SIM group); (3) 200 mg/L mSDF-1 solution + collagen scaffold (mSDF-1 group); and (4) 10® mol/L SIM +200 mg/L mSDF-1 solution + collagen scaffold (SIM + mSDF-1 group). One week after implantation, the mice were treated by injecting the same drug solution mentioned above around the scaffold once a day for two days. The specimens were harvested 6 weeks after implantation and the bone formation was evaluated