Simultaneous content determination of 14 components in Rhei Radix et Rhizoma by high performance liquid chromatography method

• Published in: Zhongguo zhongyao zazhi Vol. 42; no. 23; p. 4514
• Main Authors: Liu, Yue-Hong, Huang, Zheng-Hai, Dong, Ling, Pei, Wen-Xuan, Sun, Yu, Gao, Xiao-Yan
• Format: Journal Article
• Language: Chinese
• Published: China 01.12.2017
• Subjects: Anthraquinones - analysis; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal - analysis; Plant Roots - chemistry; Rheum - chemistry; Rhizome - chemistry; content determination; anthraquinones; tannins; Rhei Radix et Rhizoma; butanone glycosides; HPLC
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20171113.006

To establish an HPLC (high performance liquid chromatography) method for the simultaneous content determination of gallic acid, (+)-catechin, (－)-epicatechin-3-O-gallate, isolindleyin, 4-(4'-hydroxyphenyl)-2-butanone, emodin, chrysophanol, physcion, aloe-emodin, rhein, lindleyin, 4-(4'-hydroxyphenyl)-2-butanone-4'-O-β-D-(2″-O-galloyl-6″-O-cinnamoyl)-glucopyranoside, sennoside A and sennoside B in Rhei Radix et Rhizoma. The analysis was performed on Agilent Zorbax SB-C₁₈ (4.6 mm×150 mm, 5 μm) with 0.05% phosphoric acid solution (A) - acetonitrile (B) as mobile phase for gradient elution. The flow rate was 1 mL•min⁻¹, with column temperature of 40 ℃ and the wavelength was set at 268 nm. All calibration curves showed good linearity (r > 0.999 9) within the concentration range. Both the intra- and inter-day precision for 14 analytes was less than 3.1%, with the mean recovery at the range of 91.80%-104.1%. Meanwhile, quantitative determination was carried out for 10 qualified samples from Rheum palmatum and 10 qua