The Establishment of Tunica Media-induced Systemic Inflammatory Responses Animal Model
To establish animal model of tunica media-induced systemic inflammatory responses and verify the stability of established model. Tunica media of aorta was captured from C57/BL6 mouse donor, followed by the intervention of homogenization. After centrifugation, the supernatant was harvested and inject...
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Published in | Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 49; no. 4; p. 604 |
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Main Authors | , , , |
Format | Journal Article |
Language | Chinese |
Published |
China
01.07.2018
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Subjects | |
Online Access | Get more information |
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Summary: | To establish animal model of tunica media-induced systemic inflammatory responses and verify the stability of established model.
Tunica media of aorta was captured from C57/BL6 mouse donor, followed by the intervention of homogenization. After centrifugation, the supernatant was harvested and injected into the acceptor of C57/BL6 mouse to mimic the featured pathological changes induced by the exposure of tunica media with circulation. ELISA assay was used to detect the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).
The levels of TNF-α, IL-6 in the model group were significant higher than those in the control group (
<0.05). There were two peak values of both TNF-α and IL-6 identified at 3 h and 24 h after the injection of supernatant in the model group.
The exposure of tunica media to circulation may induce systemic inflammatory responses. |
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ISSN: | 1672-173X |