The Establishment of Tunica Media-induced Systemic Inflammatory Responses Animal Model

To establish animal model of tunica media-induced systemic inflammatory responses and verify the stability of established model. Tunica media of aorta was captured from C57/BL6 mouse donor, followed by the intervention of homogenization. After centrifugation, the supernatant was harvested and inject...

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Published inSichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 49; no. 4; p. 604
Main Authors Gu, Jun, Meng, Wei, Gan, Chang-Ping, Zhang, Shu
Format Journal Article
LanguageChinese
Published China 01.07.2018
Subjects
Animals
Aorta
Disease Models, Animal
Interleukin-6 - blood
Mice
Mice, Inbred C57BL
Systemic Inflammatory Response Syndrome - pathology
Tumor Necrosis Factor-alpha - blood
Tunica Media - pathology
Aortic dissection
Systemic inflammatory response
Inflammatory mediators
Tunica media
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Summary:To establish animal model of tunica media-induced systemic inflammatory responses and verify the stability of established model. Tunica media of aorta was captured from C57/BL6 mouse donor, followed by the intervention of homogenization. After centrifugation, the supernatant was harvested and injected into the acceptor of C57/BL6 mouse to mimic the featured pathological changes induced by the exposure of tunica media with circulation. ELISA assay was used to detect the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). The levels of TNF-α, IL-6 in the model group were significant higher than those in the control group ( <0.05). There were two peak values of both TNF-α and IL-6 identified at 3 h and 24 h after the injection of supernatant in the model group. The exposure of tunica media to circulation may induce systemic inflammatory responses.
ISSN:1672-173X