MicroRNA-182 modulates high glucose-induced cardiomyocyte hypertrophy via targeting Rac1

• Published in: Zhōnghuá xīnxuèguănbìng zázhì Vol. 43; no. 7; p. 619
• Main Authors: Meng, Zheying, Wang, Yu, Lin, Yanduan, Nan, Shuliang, Xu, Weiping, Hu, Bing, Shen, E
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2015
• Subjects: Animals; Cardiomyopathy, Hypertrophic - metabolism; Down-Regulation; Glucose - physiology; Mice; MicroRNAs - physiology; Myocytes, Cardiac - metabolism; Neuropeptides - metabolism; rac1 GTP-Binding Protein - metabolism; Rats, Sprague-Dawley; Transfection; Up-Regulation
• ISSN: 0253-3758

To investigate the role and signalling of microRNA(miR)-182 on regulating high glucose-induced cardiomyocyte hypertrophy. The candidates of miR which might potentially be involved on targeting Rac1 were predicted by applying bioinformatics analysis. The expression of all related candidates miRs was verified by real-time reverse transcription-PCR (RT-PCR) in cardiac tissues of db/db mice and db/m mice. Then the relationship between candidates miR and Rac1 was investigated with Pearson relevant analysis. Neonatal mice cardiomyocytes were cultured and divided into 2 groups: normal glucose group and high glucose group. The level of selected miR and Rac1 in two groups was detected by RT-PCR. Neonatal mice cardiomyocytes were then randomly divided into 4 groups: normal glucose group, selected microRNA mimics control group, high glucose group, high glucose plus selected miR mimics control group. The morphology of cardiomyocyte in each group was detected under light microscope. Furthermore, Rac1, β-MHC and α-SMA expr