Effect of WS070117M1 on chronic obstructive pulmonary disease in mice and the underling mechanisms of anti-inflammation

• Published in: Yao hsüeh hsüeh pao Vol. 50; no. 8; p. 986
• Main Authors: Cao, Shu-hua, Xuan, Ling-ling, Wang, Dong-mei, Xie, Jian-lin, Jiang, Ren-tao, Bai, Jin-ye, Wu, Song, Hou, Qi
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2015
• Subjects: Adenosine - analogs & derivatives; AMP-Activated Protein Kinases - metabolism; Animals; Bronchoalveolar Lavage Fluid; Cell Line, Tumor; Disease Models, Animal; Humans; Inflammation - drug therapy; Interleukin-1beta - metabolism; Interleukin-6 - metabolism; Interleukin-8 - metabolism; Leukocyte Count; Lipopolysaccharides; Male; Mice; Mice, Inbred BALB C; Neutrophils - cytology; Nicotiana; Pulmonary Disease, Chronic Obstructive - drug therapy; Smoke - adverse effects; Transforming Growth Factor beta1 - metabolism
• ISSN: 0513-4870

The aim of this study is to investigate the anti-inflammatory effect of the adenosine derivative N6-(3-hydroxylaniline) adenosine (WS070117M1) on cigarette smoke plus LPS (lipopolysaccharide)-induced chronic obstructive pulmonary disease (COPD) in mice and its mechanism. COPD model was established by exposing male BALB/c mice to cigarette smoke and challenged with LPS inhalation. Supernatants of bronchoalveolar lavage fluid (BALF) were harvested and IL-1β, IL-6, IL-8 and TGF-β1 levels were measured by ELISA (enzyme-linked immunesorbent assay). The number of total white blood cells and neutrophils in bronchoalveolar lavage fluid was counted separately. Lung tissue was stained with Mayer 's hematoxylin and eosin for histopathologic examination. pAMPKa protein expression and distribution of lung tissue were analyzed by immunohistochemistry method. In vitro, levels of AMPKα phosphorylation in phorbol-12- myristate-13-acetate (PMA) differentiated THP-1 cells was detected by immunohistochemistry, IL-8 level in supe